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2X Probe qPCR Master Mix

Croyez 2X Probe qPCR Master Mix is an optimized reaction mix for real-time polymerase chain reaction (qPCR) kit. This product is suitable for probe-based detection and formulated as a 2-fold premix. Reaction can be simply set up by adding the DNA template, primers, and probes. This master mix does not contain ROX reference dye; it offers great convenience and minimizes the risk of cross-contamination.
No. Size Price Qty Status
C15015-1ML 1 mL $44.00 Inquiry
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The following procedure is a general guideline for qPCR reaction. To maintain an RNase-free environment, always wear disposable gloves, and use laboratory consumables and water of nuclease-free grade during the whole experiment course.

qPCR reaction set-up:
1. Place all required reagents on ice.
Component Amount Final concentration
2X Probe qPCR Master Mix 10 μL 1X
Forward primer (10 μM) 0.8 μL 0.4 μL
Reverse primer (10 μM) 0.8 μL 0.4 μL
Probe (10 μM) 0.4 μL 0.2 μL
DNA template X μL ≦ 250 ng
Nuclease-Free H2O Y μL -
Total reaction volume 20 μL -
* See Usage Notes for additional guidelines on primer/template preparation.
2. Gently mix the reaction thoroughly to achieve uniform distribution and briefly centrifuge (2,500-3,000 rpm).
3. Thermal cycling conditions for standard qPCR
Step Cycles Temperature Time
Enzyme activation 1 95°C 5 min
Denaturation 40-45 95°C 5–15 sec
Annealing/Extension 55-65°C 30–60 sec