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T7 RNA Polymerase Transcription Buffer Set

The T7 RNA Polymerase Transcription Buffer Set contains a T7 RNA Polymerase and a set of seven 10X RNAP reaction buffers. This set of reagents was specifically designed for the selection of efficient transcription reactions. The user simply combines DNA template, NTPs, T7 RNA polymerase, and the 10X polymerase reaction buffer to proceed following reactions.
No. Size Price Qty Status
C15027-K01 1 mL*6 $360.00
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Package & Component:
Cat. Name Amount
C15027-K01 T7 RNA Polymerase (50 U/μL) 5,000 U
10X RNA Polymerase Reaction Buffer A 1 mL
10X RNA Polymerase Reaction Buffer B 1 mL
10X RNA Polymerase Reaction Buffer C 1 mL
10X RNA Polymerase Reaction Buffer D 1 mL
10X RNA Polymerase Reaction Buffer E 1 mL
10X RNA Polymerase Reaction Buffer F 1 mL
10X RNA Polymerase Reaction Buffer G 1 mL

Storage:
Stored at -20°C. Avoid repeated freeze/thaw cycles.

Manuel:
1. Below reaction mixture should be prepared under room temperature and combined in the following order:
Component Amount Final concentration
Nuclease-Free H2O X μL -
Template DNA 0.2-1 μg 10-50 ng/μL linearized DNA
10X RNA Polymerase Reaction Buffer 2 μL 1 X
ATP/UTP/CTP/GTP Mix,
10 mM each
1 μL 0.5 mM
T7 RNA Polymerase (50 U/μL) 1-2 μL -
RNase inhibitor (optional) 0.5 μL 1 U/μL
Total reaction volume 20 μL -
2. Incubate at 37°C for 2-16 hours.
3. Above reaction mixture may be scaled up or down proportionately.

Notes:
Transcription reaction should be performed under RNase free condition. Use nuclease-free tubes, reagents, and water to avoid RNase contamination. Also, wear gloves when working with RNA.