Croyez GMP ® IL-15 (Interleukin-15), Human
Interleukin-15 (IL-15) is a cytokine with structural similarity to Interleukin-2 (IL-2).
Like IL-2, IL-15 binds to and signals through a complex composed of IL-2/IL-15
receptor beta chain (CD122) and the common gamma chain (gamma-C, CD132).
IL-15 is secreted by mononuclear phagocytes (and some other cells) following
infection by virus(es). This cytokine induces cell proliferation of natural killer cells; cells of the innate immune system whose principal role is to kill virally infected cells.
✔️ High Bioactivity
✔️ Low cost
✔️ Low endotoxin
✔️ High purity
NWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVENLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIV
QMFINTS with polyhistidine tag at the C-terminus
UnitProt ID
P40933
Species of Origin
Human
Expression System
Escherichia coli
Endotoxin level
<0.05 EU per 1 μg of the protein by the LAL method.
Activity
Measure by its ability to induce proliferation in CTLL-2 cells. The ED50 for this effect is < 3 ng/mL.
The specific activity of recombinant Croyez GMP® human IL-15 is approximately 2.5 x 107 IU/mg, which is calibrated against the human IL-15 WHO International Standard (NIBSC code: 95/554).
Purity
>98% as determined by SDS-PAGE analysis.
Form
Lyophilized
Storage Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.
Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Stability & Storage
This product is stable after storage at:
• -20°C for 12 months in lyophilized state from date of receipt.
• -20°C or -80°C for 6 months under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Shipping conditions
Blue ice
Background Information
Interleukin-15 (IL-15) is a cytokine with structural similarity to Interleukin-2 (IL-2), both cytokines can activate CAR T cells and NK cells in proliferation or maintainence. Croyez offers GMP IL-15 which is prodcued in Taiwan facility, regarding the bioacitivty, we are confident in competing with Peprotech, R&D system, Thermp, Miltenyi.
Quality Statement
Croyez GMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP-certified facility.
The processes include:
● Animal-free reagent and laboratory
● Manufactured and tested under GMP guideline
● Testing and traceability of raw material
● Records of the maintenance and equipment calibration
● Personnel training records
● Batch-to-batch consistency
● Documentation of QA control and process changes
● Manufactured and tested under an ISO 13485:2016 certified quality management system
● Stability monitor of product shelf-life
Quality Assurance
At Croyez, we are committed to providing our valued customers with detailed product analytics to assist in their research endeavors. Our Certificate of Analysis includes the following lot-specific details:
● SDS-PAGE analysis and endotoxin level evaluation conducted on bulk QC lots.
● Lot-specific bioassay results ensuring compliance with established parameters, including microbial testing meeting USP <71> standards.
● Mycoplasma detection via PCR analysis.
We believe in transparency and strive to empower our customers with comprehensive information to aid in their decision-making process.
Measure by its ability to induce proliferation in CTLL-2 cells. The ED50 for this effect is < 3 ng/ mL.
The specific activity of recombinant human IL-15 is approximately 2.5 x 107 IU/ mg.
To demonstrate lot-to-lot consistency of GMP IL-15, the activity of three separate lots was examined and plotted on the same graph.
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