Guidelines for Selecting Recombinant Proteins, Cytokines and Growth Factors
2023-12-22
Recombinant proteins, especially cytokines and growth factors, play a crucial role in various applications, from cell culture in biotechnology labs to bioprocessing and advanced therapies. Selecting the right recombinant proteins, including an array of cytokines, involves careful consideration of several key factors. Here are essential guidelines to help you make informed decisions:
The choice of different systems for protein expression can impact the characteristics and marketability of the protein. For example, utilizing the E. coli system allows for a simpler protein expression, leading to reduced costs and achieving higher protein yields in a shorter time frame. On the other hand, Croyez also provides proteins derived from HEK293 cells, offering distinct advantages such as native post-translational modifications (PTMs) and glycosylation. These features enhance the stability and activity of the proteins, contributing to their overall quality.
Our range of recombinant growth factors and cytokines comes in formulations that are entirely free of bovine serum albumin (BSA), ensuring a solution that aligns with the highest standards of quality and purity.
Ensure the product's quality by checking data on purity, endotoxin concentration, and bioactivity. Reliable companies subject their proteins, including cytokines, to rigorous quality control. Bioassays, measured in IU/ mg or ED50, provide insights into functional activity. Explore Croyez's selection of bioactive recombinant proteins, including an extensive range of cytokines, supported by comprehensive data on their applications and functional attributes.
Thoroughly review the information provided by the company, including bioactivity, purity (>95% is ideal), endotoxin concentration, and validation through SDS-PAGE. This information reflects the depth of validation performed, ensuring the product meets the required standards.
If your research has the potential for clinical applications, consider Croyez Good Manufacturing Practice (GMP)-grade products to facilitate a smooth transition to the clinic. Research Use Only (RUO) products are suitable for non-clinical purposes. You can also check on Croyez's GMP quality statement for making better decisions.
In conclusion, choosing the suitable recombinant proteins, including Cytokines and Growth Factors, involves comprehensive consideration of protein source, formulation, bioactivity, and product information. Recombinant proteins, including Cytokines and Growth Factors, from reliable suppliers offer advantages in terms of source, stability, and activity. Remember to assess the specific needs of your research, especially in cytokine-related studies and test shortlisted products in parallel before making a final decision. However, finding the most suitable products, including a comprehensive array of cytokines, doesn't have to be a challenging task – feel free to contact our experts at Croyez for personalized recommendations and expert guidance.
1. Protein Source
The expression system used for generating recombinant proteins significantly influences the final product. The source of the protein, whether from E. coli, yeast, or human cells like HEK293, impacts post-translational modifications (PTMs) and glycosylation.The choice of different systems for protein expression can impact the characteristics and marketability of the protein. For example, utilizing the E. coli system allows for a simpler protein expression, leading to reduced costs and achieving higher protein yields in a shorter time frame. On the other hand, Croyez also provides proteins derived from HEK293 cells, offering distinct advantages such as native post-translational modifications (PTMs) and glycosylation. These features enhance the stability and activity of the proteins, contributing to their overall quality.
2. Formulation
Our range of recombinant growth factors and cytokines comes in formulations that are entirely free of bovine serum albumin (BSA), ensuring a solution that aligns with the highest standards of quality and purity.
3. Bioactivity and Applications
Ensure the product's quality by checking data on purity, endotoxin concentration, and bioactivity. Reliable companies subject their proteins, including cytokines, to rigorous quality control. Bioassays, measured in IU/ mg or ED50, provide insights into functional activity. Explore Croyez's selection of bioactive recombinant proteins, including an extensive range of cytokines, supported by comprehensive data on their applications and functional attributes.
4. Product Information
Thoroughly review the information provided by the company, including bioactivity, purity (>95% is ideal), endotoxin concentration, and validation through SDS-PAGE. This information reflects the depth of validation performed, ensuring the product meets the required standards.
5. GMP Considerations
If your research has the potential for clinical applications, consider Croyez Good Manufacturing Practice (GMP)-grade products to facilitate a smooth transition to the clinic. Research Use Only (RUO) products are suitable for non-clinical purposes. You can also check on Croyez's GMP quality statement for making better decisions.In conclusion, choosing the suitable recombinant proteins, including Cytokines and Growth Factors, involves comprehensive consideration of protein source, formulation, bioactivity, and product information. Recombinant proteins, including Cytokines and Growth Factors, from reliable suppliers offer advantages in terms of source, stability, and activity. Remember to assess the specific needs of your research, especially in cytokine-related studies and test shortlisted products in parallel before making a final decision. However, finding the most suitable products, including a comprehensive array of cytokines, doesn't have to be a challenging task – feel free to contact our experts at Croyez for personalized recommendations and expert guidance.