GFP mRNA ( m1ψ substitution )
The GFP mRNA is designed for high Green Fluorescent Protein expression, serving as a transfection efficiency control.The protein emits bright green fluorescence (505/525nm), facilitating mRNA delivery tracking.GFP, a widely used reporter gene, allows direct visualization with standard filters. In mRNA form, it offers rapid transient expression without nuclear uptake, proportional to mRNA quantity.Croyez’s GFP mRNA is produced through in vitro transcription, featuring a Cap1-modified 5' end, a poly(A) tail at the 3' end, and modified nucleotides to reduce immune response.These enhancements ensure stability and performance, allowing the mRNA to function like natural mature mRNAs in cells. |
mRNA Length |
1332 nt |
|
Base Composition |
N1-Me-pUTP (N1-mψ) |
|
Concentration |
1.0 mg/ mL |
|
Cap Modification |
Cap 1 structure |
|
Poly A Tail |
Yes |
|
Form |
Liquid |
|
Buffer |
1 mM sodium citrate buffer, pH 6.4. |
|
Storage |
Products can be stored at -80°C or below.We recommend to aliquot the mRNA solution for a better storage. Avoid repeated freeze/thaw cycles. |
|
Shipping |
The products are shipped on dry ice and should be avoided for freeze-thaw cycles. |
|
Application |
Reporter Genes |
|
Shipping Conditions |
Dry ice |
.jpg)
Figure 1.
GFP mRNA (m1 ψ substitution) was analyzed on a 2% E-Gel.
Lane 1: Marker, RNA ladder (cat no: CR00004)
Lane 2: GFP mRNA (m1 ψ substitution)
.jpg)
Figure 2.
GFP mRNA (m1 ψ substitution) was analyzed on a 1% TAE agarose gel at 100 V for 30 minutes.
Lane 1: Marker,High Range RNA ladder (cat no: CR00005).
Lane 2: GFP mRNA (m1 ψ substitution)
Figure 3.
Capillary Electrophoresis
GFP mRNA (m1 ψ substitution) was analyzed by capillary electrophoresis
Figure 4.
293T cells (0.8 × 10⁵ cells per well, 24-well plate) were transfected with 1 µg of GFP mRNA (m1 ψ substitution) using the EndoSafe mRNA Transfection Kit (C15053-K01).
