dCas9-KRAB mRNA (m1ψ substitution)
dCas9-KRAB mRNA encodes the dCas9-KRAB fusion protein, a tool for gene silencing and transcriptional repression.The dCas9 (dead Cas9) component targets DNA precisely without cutting, while the KRAB (Krüppel-associated box) domain recruits complexes to inhibit gene expression. Delivered as mRNA, dCas9-KRAB ensures rapid, high transient expression without nuclear uptake, making it ideal for research in gene function, genetic diseases, and therapeutic development.Croyez’s dCas9-KRAB mRNA is produced through in vitro transcription, featuring a Cap1-modified 5' end, a poly(A) tail at the 3' end, and modified nucleotides to reduce immune response. These enhancements ensure stability and performance, allowing the mRNA to function like natural mature mRNAs in cells.
| mRNA Length | 4982 nt |
| Base Composition | Unmodified bases |
| Concentration | 1.0 mg/ mL |
| Cap Modification | Cap 1 structure |
| Poly A Tail | Yes |
| Form | Liquid |
| Buffer | 1 mM sodium citrate buffer, pH 6.4. |
| Storage | Products can be stored at -80°C or below. We recommend to aliquot the mRNA solution for a better storage. Avoid repeated freeze/thaw cycles. |
| Shipping | The products are shipped on dry ice and should be avoided for freeze-thaw cycles. |
| Application | Gene function research, genetic disease studies, therapeutic development, genetic modification. |
| Shipping Conditions | Dry ice |
⮕dCas9-KRAB mRNA
ARTICLE：
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Figure 1.
dCas9-KRAB mRNA (m1 ψ substitution) was analyzed on a 2% E-Gel.
Lane 1: Marker,RNA ladder (cat no: CR00004)
Lane 2: dCas9-KRAB mRNA (m1 ψ substitution)
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Figure 2.
100 ng RNA + 4 mL RNA loading dye+ 1 mL novel juice, 5 min , 65℃ loading 6 mL to 1% TAE agarose gel, 120 V 30 min
Lane 1: Marker, RNA ladder (cat no: CR00004)
Lane 2: dCas9-KRAB mRNA (m1 ψ substitution)
