The GFP mRNA is designed for high Green Fluorescent Protein expression, serving as a transfection efficiency control. The protein emits bright green fluorescence (505/525nm), facilitating mRNA delivery tracking. GFP, a widely used reporter gene, allows direct visualization with standard filters. In mRNA form, it offers rapid transient expression without nuclear uptake, proportional to mRNA quantity. Croyez’s GFP mRNAs was generated through in vitro transcription, and these mRNAs are then fortified at their 5' end by modified nucleotide capping, known as Cap1. To mimic the characteristics of fully processed mature mRNAs, we incorporate a poly(A) tail at the 3' end and optimize the mRNAs to enhance stability and overall performance. This ensures that the mRNAs function similarly to naturally occurring mature mRNAs in cells.