Nuclease
Nuclease is a genetically engineered endonuclease from Serratia marcescens, it is produced and purified from E. coli. The enzyme is a non-specific nuclease, which effectively degrades single-stranded, double-stranded, linear, and circular DNA and RNA over a wide temperature and pH range.
Source:
Escherichia coli
Purity:
>98% as determined by SDS-PAGE. Ni-NTA chromatography
Unit Definition:
One unit of Nuclease is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 min, which corresponds to complete digestion of 37 μg of DNA. (Note that 1 KU=1,000 U)
Storage Buffer:
Nuclease is supplied in 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 2 mM MgCl₂ and 50% glycerol.
Stability & Storage:
This product is stable after storage at:
● -20°C for -80°Clong-term storage under sterile conditions.
Avoid repeated free-thaw cycles.
Manuel:
Note:
For safety, use protective equipment to avoid eye, face, and skin contact.
The amount of Nuclease required for nucleic acid hydrolysis can be adjusted as appropriate.
Shipping Conditions:
Blue ice
Escherichia coli
Purity:
>98% as determined by SDS-PAGE. Ni-NTA chromatography
Unit Definition:
One unit of Nuclease is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 min, which corresponds to complete digestion of 37 μg of DNA. (Note that 1 KU=1,000 U)
Storage Buffer:
Nuclease is supplied in 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 2 mM MgCl₂ and 50% glycerol.
Stability & Storage:
This product is stable after storage at:
● -20°C for -80°Clong-term storage under sterile conditions.
Avoid repeated free-thaw cycles.
Manuel:
| Sample Type | Sample Amount | Lysis Buffer Amount (RIPA buffer) |
Nuclease |
| E. coli | Pellet from 500 μL culture |
100 μL | 1-2 μL |
| Cell | 106 -107 | 100 μL | 1-2 μL |
Note:
For safety, use protective equipment to avoid eye, face, and skin contact.
The amount of Nuclease required for nucleic acid hydrolysis can be adjusted as appropriate.
Shipping Conditions:
Blue ice