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T7 RNA Polymerase Transcription Buffer Set

The T7 RNA Polymerase Transcription Buffer Set contains a T7 RNA Polymerase and a set of seven 10X RNAP reaction buffers. This set of reagents was specifically designed for the selection of efficient transcription reactions. The user simply combines DNA template, NTPs, T7 RNA polymerase, and the 10X polymerase reaction buffer to proceed following reactions.
No. Size Price Qty Status
C15027-K01 1 mL*6 $360.00 Inquiry
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Package & Component:
Cat. Name Amount
C15027-K01 T7 RNA Polymerase (200 U/μL) 10,000 U
10X RNA Polymerase Reaction Buffer A 1 mL
10X RNA Polymerase Reaction Buffer B 1 mL
10X RNA Polymerase Reaction Buffer C 1 mL
10X RNA Polymerase Reaction Buffer D 1 mL
10X RNA Polymerase Reaction Buffer E 1 mL
10X RNA Polymerase Reaction Buffer F 1 mL
10X RNA Polymerase Reaction Buffer G 1 mL

Storage:
Stored at -20°C. Avoid repeated freeze/thaw cycles.

Manuel:
1. Below reaction mixture should be prepared under room temperature and combined in the following order:
Component Amount Final concentration
Nuclease-Free H2O X μL -
Template DNA 0.5-1 μg -
10X RNA Polymerase Reaction Buffer 2 μL 1 X
ATP (100 mM) 2 μL 10 mM
UTP (100 mM) 2 μL 10 mM
CTP (100 mM) 2 μL 10 mM
GTP (100 mM) 2 μL 10 mM
T7 RNA Polymerase (200 U/μL) 1 μL -
RNase inhibitor (optional) 0.5 μL 1 U/μL
Total reaction volume 20 μL -
2. Incubate at 37°C for 30 minutes to 2 hours.
3. Above reaction mixture may be scaled up or down proportionately.

Notes:
1. Transcription reaction should be performed under RNase free condition. Use nuclease-free tubes, reagents, and water to
    avoid RNase contamination. Also, wear gloves when working with RNA. .
2. To obtain optimal condition, NTP concentration can be titrated between 10 – 15 mM.
3. The volume of T7 RNA Polymerase can be titrated between 1-2 μL in the IVT reaction to optimize your assay.