Products

Inorganic Pyrophosphatase (Yeast)

Inorganic pyrophosphate (PPi) is generated as a reaction byproduct in many biosynthetic reactions which utilize ATP, including in vitro transcription and DNA polymerization. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate (P2O7-4 + H2O + PPase 2HPO4-2). PPase requires divalent metal cation (Mg2+) for its enzymatic activity.
No. Size Price Qty Status
C15026-10U 10 U -
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Source:
Escherichia coli
 
Purity:
>98% as determined by SDS-PAGE. Purified by Ni-NTA chromatography.
 
Unit Definition:
One unit is defined as the amount of the enzyme hydrolysis 1 μmol of inorganic pyrophosphate in 1 minutes at 25°C.
 
Reaction Condition:
Standard reaction: 100 mM Tris-HCl (pH 7.2), 2 mM MgCl2 and 2 mM inorganic pyrophosphate.
 
Storage Buffer:
This enzyme is supplied in 20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT and 50% glycerol.

Storage:
Stored at -20°C. Avoid repeated freeze/thaw cycles.

Application:
Higher RNA yields in transcription reaction

Notes:
Inorganic Pyrophosphatase (Yeast) requires divalent metal cation (Mg2+) for its enzymatic activity. This enzyme is widely used in RNA IVT reaction.