Method
Sandwich ELISA

Principle of Assay
HRV 3C Protease ELISA kit is used to detect HRV 3C Protease in samples by sandwich ELISA method. This assay uses microplate pre-coated with mouse anti-HRV 3C Protease monoclonal antibody to the solid phase. HRV 3C Protease in the samples conjugates on solid phase and then react with the HRP conjugated mouse anti-HRV 3C Protease monoclonal antibody. Subsequent wash steps will residual unbound antibody. After incubation with substrate solution, the reaction is determined by the absorbance at 450 nm. Quantification of HRV 3C Protease level is accomplished by comparing the absorbance with standards curve.

Platform
Pre-coated 96 Microplate (12 x 8 well strips)

Assay Time
2 hours

Sensitivity
0.057 ng/mL

Detection Range
1.5625 -100 ng/mL

Reactivity
HRV 3C Protease

Conjugation
HRP

Alternate Names
Human Rhinovirus 3C Protease, Levlfqgp site protease, PreScission Protease 

Components

• HRV 3C Protease ELISA plate
• Standard
• Standard reconstitution buffer
• Standard & Sample diluent buffer
• HRP-antibody conjugate
• HRP-antibody conjugated diluent buffer
• 20 X wash buffer
• TMB
• Stop solution
• Microplate sealing film

Storage
Store at 2-8°C. Do not use past expiration date.

Usage
For Research Use Only
 

---

Background Information
HRV 3C Protease is a recombinant cysteine protease from human rhinovirus 3C. It can recognize and cleave the sequence: : Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro (LEVLFQ↓GP). This protease is used to remove fusion tags from recombinant proteins with the specific cleavage site. Therefore, detection of HRV 3C Protease is important for evaluating the level of HRV 3C Protease in samples.