Streptavidin-HRP
Streptavidin is a 53 kDa homotetramer isolated from Streptomyces avidinii.
Because of its strong non-covalent interaction with biotin, streptavidin can be used to detect and isolate biotinylated proteins. Conjugation of horseradish peroxidase (HRP) to streptavidin is obtained by cross linking the amino groups on streptavidin with the carbohydrate groups on HRP. Streptavidin-HRP is typically used biotinylated antibodies or proteins.
Because of its strong non-covalent interaction with biotin, streptavidin can be used to detect and isolate biotinylated proteins. Conjugation of horseradish peroxidase (HRP) to streptavidin is obtained by cross linking the amino groups on streptavidin with the carbohydrate groups on HRP. Streptavidin-HRP is typically used biotinylated antibodies or proteins.
Conjugation:
Horseradish peroxidase (HRP), to streptavidin
Form:
Liquid
Storage buffer:
Phosphate Buffered Saline containing 50% glycerol and 0.01% ProCline 300, pH 7.4.
Storage:
Store at 4°C for 6 months.
Store at -20°C for 1 year. Avoid repeated freeze/thaw cycles.
Suggestion: Divide antibody into several vials. Keep only vials for usage at 4°C.
Application:
ELISA, WB, IHC
Application Note:
Horseradish peroxidase is conjugated to the streptavidin tetramer at about 1:1 molar ratio.
Optimal titers for the applications should be determined by the researcher.
Manual:
Note: Application concentration may be various determined by the end user.
Shipping Conditions:
Blue ice
Horseradish peroxidase (HRP), to streptavidin
Form:
Liquid
Storage buffer:
Phosphate Buffered Saline containing 50% glycerol and 0.01% ProCline 300, pH 7.4.
Storage:
Store at 4°C for 6 months.
Store at -20°C for 1 year. Avoid repeated freeze/thaw cycles.
Suggestion: Divide antibody into several vials. Keep only vials for usage at 4°C.
Application:
ELISA, WB, IHC
Application Note:
Horseradish peroxidase is conjugated to the streptavidin tetramer at about 1:1 molar ratio.
Optimal titers for the applications should be determined by the researcher.
Manual:
| Application | Dilution factor |
| ELISA | 1:5000-20000 |
| WB | 1:1000-10000 |
| IHC | 1:200-500 |
Shipping Conditions:
Blue ice