Annexin V-FAM + PI Apoptosis Detection Reagent

Croyez fluorescent dye (FAM) conjugated Annexin V is highly purified product. During early apoptosis, cells will translocate membrane phosphatidylserine (PS) from the inner face of the membrane to the cell surface. Propidium iodide (PI) is a common fluorescent dye to detect DNA. It can be used in flow cytometry to evaluate the cell cycle and cell viability during apoptosis. The product can be used in one-step staining procedure without wash step within 20 minutes.
No. Size Price Qty Status
C08004-K01 25 rxns $120.00 Inquiry
C08004-K02 50 rxns $180.00 Inquiry
C08004-K03 100 rxns $300.00 Inquiry
The price does not include shipping fee and tax. Order Request Quote
  C08004-K01 C08004-K02 C08004-K03
Annexin V-FAM 1 x 0.125 mL 1 x 0.25 mL 1 x 0.5 mL
PI 1 x 0.125 mL 1 x 0.25 mL 1 x 0.5 mL
10X Binding Buffer 1 x 2 mL 2 x 2 mL 3 x 2 mL
Store at 4°C in the dark.
All reagents are stable for one year under proper storage conditions.
1. Collect 1-5x105 cells in the flow tube by centrifugation.
2. Wash cells in 2 mL cold phosphate-buffered saline (PBS) and collect by centrifugation.
3. Re-suspend cell in 500 μL of 1X Binding Buffer.
4. Add 5 μL of Annexin V-FAM and 5 μL of PI, gently mix the cells and incubate for 15-20 minutes at RT in the dark.
5. After incubation, the samples should be kept on ice and perform flow cytometry using filters appropriated for fluorescein 
   (FITC, corresponding to Annexin V-FAM).