IL-2 (Interleukin-2), Human
Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes.
Sequence
MAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIV
LELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT with polyhistidine tag at the C-terminus
UnitProt ID:
P60568
Source
Escherichia coli
Endotoxin Test
<0.1 EU per 1 μg of the protein by the LAL method.
Activity
Measure by its ability to induce proliferation in CTLL-2 cells. The ED50 for this effect is <0.2 ng/mL.
The specific activity of recombinant human IL-2 is approximately >2.5 x 107 IU/mg.
Measure by its ability to induce proliferation in NK cells. The ED50 for this effect is <46 ng/mL.
Purity
>98% as determined by SDS-PAGE.
Form
Lyophilized
Storage Buffer
Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Stability & Storage
This product is stable after storage at:
• -20°C for 12 months in lyophilized state from date of receipt.
• -20°C or -80°C for 6 months under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Shipping Conditions:
Blue ice
MAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIV
LELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT with polyhistidine tag at the C-terminus
UnitProt ID:
P60568
Source
Escherichia coli
Endotoxin Test
<0.1 EU per 1 μg of the protein by the LAL method.
Activity
Measure by its ability to induce proliferation in CTLL-2 cells. The ED50 for this effect is <0.2 ng/mL.
The specific activity of recombinant human IL-2 is approximately >2.5 x 107 IU/mg.
Measure by its ability to induce proliferation in NK cells. The ED50 for this effect is <46 ng/mL.
Purity
>98% as determined by SDS-PAGE.
Form
Lyophilized
Storage Buffer
Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.
Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Stability & Storage
This product is stable after storage at:
• -20°C for 12 months in lyophilized state from date of receipt.
• -20°C or -80°C for 6 months under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Shipping Conditions:
Blue ice