Mito-GFP mRNA ( m1ψ substitution )
Mito-GFP mRNA is designed for live-cell mitochondrial labeling and tracking. By encoding a GFP reporter fused with a mitochondrial targeting signal (MTS), it enables precise mitochondrial localization and rapid, transient visualization of mitochondrial dynamics such as movement, fusion, and fission. This tool supports studies of mitochondrial function, disease mechanisms, mitophagy, and drug screening applications. Synthesized with a Cap 1 structure, poly(A) tail, and m1ψ modification, it is optimized for stable and efficient expression in cell-based research.
ARTICLE：
➡︎Mito-GFP mRNA: A Next-Generation Tool for Live-Cell Mitochondrial Imaging
Figure1.
0.6 x105 293T cells were transfected transfected with 1 mg Mito-GFP mRNA (m1 ψ substitution, CR00047) by EndoSafe reagent. After 18 h, cells were stained with Hoechst 33342 and Mito-Traker Red FM for confocal microscope image aquirement.
Figure 2:
Mito-GFP mRNA (m1 ψ substitution) was analyzed on a 2% E-Gel.
Lane 1: Marker, RNA ladder (cat no: CR00004).
Lane 2: Mito-GFP mRNA (m1 ψ substitution)
Figure 3:
Mito-GFP mRNA (m1 ψ substitution) was analyzed on a 1% TAE agarose gel at 100 V for 30 minutes.
Lane 1: Marker, RNA ladder (cat no: CR00004).
Lane 2: Mito-GFP mRNA (m1 ψ substitution)
Figure 4:
Capillary Electrophoresis
High-Resolution capillary electrophoresis separation of Mito-GFP mRNA
|
mRNA Length |
1487 nt |
|
Base Composition |
N1-Me-pUTP (N1-mψ) |
|
Concentration |
1.0 mg/ mL |
|
Cap Modification |
Cap 1 structure |
|
Poly A Tail |
Yes |
|
Form |
Liquid |
|
Buffer |
1 mM sodium citrate buffer, pH 6.4. |
|
Storage |
Products can be stored at -80°C or below. We recommend to aliquot the mRNA solution for a better storage. Avoid repeated freeze/thaw cycles. |
|
Shipping |
The products are shipped on dry ice and should be avoided for freeze-thaw cycles. |
|
Application |
Reporter Genes |
|
Shipping Conditions |
Dry ice |
