Ultra High Range RNA Ladder
The Ultra High Range RNA ladder, comprising four single-stranded RNAs (6k, 9k, 12k and 15k), is synthetically generated through in vitro transcription from a blend of linear DNA templates. This ladder serves as an effective tool for determining the size of single-stranded RNAs in native agarose gel electrophoresis. Visualization can be achieved using UV light post-ethidium bromide staining or nucleic acid safety dye staining. Notably, it acts as a reliable ssRNA size standard on native agarose gels.
Quality Control Testing
The banding pattern of RNA ladder on 0.7% TAE or TBE agarose gels shows clear identifiable bands at each fragment, when stained with nucleic acid safety dye under UV light.
Storage Buffer
1 mM sodium citrate buffer (pH 6.4)
Storage & Stability
This product is stable for 10 months after storage at -80°C and avoid repeated freeze/thaw cycles.
Handling Instruction
For optimal storage, aliquot the reagent into smaller quantities and store at recommended temperature.
Please promptly retrieve the required portion and return it to the appropriate storage temperature.
Recommended to Use
Ladder preparation for Electrophoresis
1. Mix 1 volume of RNA ladder with 3 volumes of 2X RNA Loading Dye.
2. Incubate at 65°C for 5 minutes.
3. Immediately place it on ice for 1-2 minutes
4. Load 0.13 μL of the prepared ladder for every mm of gel lane width (e.g., 1 μL for an 8 mm lane).
Applications
Electrophoresis
Note
1. To avoid ribonuclease contamination, using RNase-free water is necessary for sample dilution.
2. We do not recommend use of these markers as a quantitative standard.
Shipping Conditions
Dry ice
The banding pattern of RNA ladder on 0.7% TAE or TBE agarose gels shows clear identifiable bands at each fragment, when stained with nucleic acid safety dye under UV light.
Storage Buffer
1 mM sodium citrate buffer (pH 6.4)
Storage & Stability
This product is stable for 10 months after storage at -80°C and avoid repeated freeze/thaw cycles.
Handling Instruction
For optimal storage, aliquot the reagent into smaller quantities and store at recommended temperature.
Please promptly retrieve the required portion and return it to the appropriate storage temperature.
Recommended to Use
Ladder preparation for Electrophoresis
1. Mix 1 volume of RNA ladder with 3 volumes of 2X RNA Loading Dye.
2. Incubate at 65°C for 5 minutes.
3. Immediately place it on ice for 1-2 minutes
4. Load 0.13 μL of the prepared ladder for every mm of gel lane width (e.g., 1 μL for an 8 mm lane).
Applications
Electrophoresis
Note
1. To avoid ribonuclease contamination, using RNase-free water is necessary for sample dilution.
2. We do not recommend use of these markers as a quantitative standard.
Shipping Conditions
Dry ice
Figure 1:
1% E-gel
Figure 2:
100 ng RNA , 1% E-gel
Figure 3:
Agarose gel 1 μL / lane 0.7 % TAE agarose gel./100 ng RNA , 0.7% TAE agarose gel
Figure 4:
Capillary electrophoresis
High-Resolution capillary electrophoresis
separation of Ultra High Range RNA ladder
