GFP mRNA LNP
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GFP mRNA_LNP is a high-performance lipid nanoparticle (LNP) system designed for efficient intracellular delivery and expression of mRNA. The formulation contains GFP-encoding mRNA, enabling rapid and reliable fluorescence expression across a wide range of applications, including cell transfection and delivery validation, transfection efficiency monitoring, formulation optimization, and in vivo targeted delivery and tracking.
1332 nt
Base Composition:
N1-Me-pUTP (N1-mψ)
Concentration:
200 μg/mL
Cap Modification:
Cap 1 structure
Poly A Tail:
Yes
Form:
Liquid
Buffer:
Tris Sucrose buffer, pH 7.4.
Storage:
Products can be stored at -80°C or below.
We recommend to aliquot the mRNA solution for a better storage. Avoid repeated freeze/thaw cycles.
Shipping:
The products are shipped on dry ice and should be avoided for freeze-thaw cycles.
Application:
Reporter Genes
Shipping Conditions:
Dry ice
Figure 1.
293T cells (0.5 x 10⁵ cells per well, 24-well plate) were transfected with 100 ng of GFP LNP-mRNA.
Figure 2.
Jurkat (2x10⁵ cells per well, 24-well plate) were transfected with 100 ng of GFP LNP-mRNA.
➡︎ Customer Feedback- GFP mRNA-LNP
Cell line : BT-549,HCC1143,HCC1395,MDA-MB-231,KE-37,U87,T98G,LN229
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| BT-549 cells (2.5 × 10⁴ cells per well, 24-well plate) were transfected with 250 ng of GFP LNP-mRNA for 24 hours. | BT-549 cells (2.5 × 10⁴ cells per well, 24-well plate) were transfected with 400 ng of GFP LNP-mRNA for 48 hours. |
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| HCC1143 cells (3.5 × 10⁴ cells per well, 24-well plate) were transfected with 225 ng of GFP LNP-mRNA and incubated for 24 hours. | HCC1143 cells (3.5 × 10⁴ cells per well, 24-well plate) were transfected with 400 ng of GFP LNP-mRNA and incubated for 24 hours. |
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HCC1395 cells (4 × 10⁴ cells per well, 24-well plate) were transfected with 250 ng of GFP LNP-mRNA and incubated for 6 hours. |
HCC1395 cells (4 × 10⁴ cells per well, 24-well plate) were transfected with 400 ng of GFP LNP-mRNA and incubated for 48 hours. |
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MDA-MB-231 cells (3 × 10⁴ cells per well, 24-well plate) were transfected with 400 ng of GFP LNP-mRNA and incubated for 6 hours. |
MDA-MB-231 cells (3 × 10⁴ cells per well, 24-well plate) were transfected with 400 ng of GFP LNP-mRNA and incubated for 72 hours. |
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KE-37 cells (5 × 10⁵ cells per well) were transfected with 200 ng of GFP LNP-mRNA and incubated for 3 days. |
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LN229 cells (4 × 10⁴ cells per well, 12-well plate) were treated with GFP mRNA LNP at 100 ng and incubated for 24 hours. |
LN229 cells (4 × 10⁴ cells per well, 12-well plate) were treated with GFP mRNA LNP at 200 ng and incubated for 24 hours. |
LN229 cells (4 × 10⁴ cells per well, 12-well plate) were treated with GFP mRNA LNP at 200 ng and incubated for 72 hours. |
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T98G cells (4 × 10⁴ cells per well, 12-well plate) were treated with GFP mRNA LNP at 200 ng and incubated for 72 hours. |
T98G cells (4 × 10⁴ cells per well, 12-well plate) were treated with GFP mRNA LNP at 250 ng and incubated for 48 hours. |
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U87 cells (4 × 10⁴ cells per well, 12-well plate) were treated with GFP mRNA LNP at 150 ng and incubated for 24 hours. |
U87 cells (4 × 10⁴ cells per well, 12-well plate) were treated with GFP mRNA LNP at 150 ng and incubated for 48 hours. |
