GFP Self-Amplifying RNA / GFP saRNA
GFP Self-Amplifying RNA expresses blue fluorescent protein in cells, emitting bright green fluorescence (505/525nm). It’s used for visualizing cellular processes, gene expression analysis, protein localization, and multiplex imaging with other markers. As a self-amplifying RNA, it provides enhanced and prolonged protein expression with lower doses compared to traditional mRNA, making it highly efficient for applications like live cell tracking and gene editing research, where extended observation periods are beneficial. This transient system is ideal for experiments requiring temporary but robust protein expression.
Croyez’s GFP saRNA was generated through in vitro transcription, and these mRNAs are then fortified at their 5' end by modified nucleotide capping, known as Cap1. To mimic the characteristics of fully processed mature mRNAs, we incorporate a poly(A) tail at the 3' end and optimize the mRNAs to enhance stability and overall performance. This ensures that the mRNAs function similarly to naturally occurring mature mRNAs in cells.
.jpg)
Figure 1:
saRNA GFP was analyzed on a 1% E-Gel.
Lane 1: Marker: High Range RNA Ladder (cat no:CR00005-50UL), Lane 2: saRNA GFP
.jpg)
Figure 2:
saRNA GFPwas analyzed on a 1% TAE agarose gel at 100 V for 30 minutes.
Lane 1: Marker: High Range RNA Ladder (cat no:CR00005-50UL), Lane 2: saRNA GFP
Lane 1: Marker: High Range RNA Ladder (cat no:CR00005-50UL), Lane 2: saRNA GFP
Figure 3:
saRNA GFP was analyzed by capillary electrophoresis.
Figure 4:
0.6 x 10⁵BHK21 cells were transfected with 500 ng saRNA by EndoSafe transfection reagent. Cells were subculture with 3 days intervals.
Figure 5.
saRNA GFP / LNP transfection in BHK21 cells
BHK21 cells were incubated with 100 ng samRNA GFP/LNP for 18 hours. After changing to fresh growth medium, the cells were monitored for GFP expression and subcultured until 34 days.
Figure 6.
saRNA GFP / LNP transfection in 293T cells
293T cells were incubated with 100 ng samRNA GFP/LNP for 18 hours. After changing to fresh growth medium, the cells were monitored for GFP expression and subcultured until 34 days.
Figure 7.
Primary T cells transfected with CROYEZ GFP-saRNA via LNPs demonstrate detectable GFP reporter expression across different RNA doses.RNA dose: 4μg/1E+06 cells/RNA dose: 8μg/1E+06 cells.
.png)
Figure 8.
Primary T cells transfected with CROYEZ GFP-saRNA via LNPs demonstrate detectable GFP reporter expression across different RNA doses.Microscopy/Confocal.
mRNA Length:
8476 nt
Base Composition:
Unmodified bases
Concentration:
1.0 mg/ mL
Cap Modification:
Cap 1 structure
Poly A Tail:
Yes
Form:
Liquid
Buffer:
1 mM sodium citrate buffer, pH 6.4.
Storage:
Products can be stored at -80°C or below.
We recommend to aliquot the mRNA solution for a better storage. Avoid repeated freeze/thaw cycles.
Shipping:
The products are shipped on dry ice and should be avoided for freeze-thaw cycles.
Application:
Reporter Genes
Shipping Conditions:
Dry ice
.png)
8476 nt
Base Composition:
Unmodified bases
Concentration:
1.0 mg/ mL
Cap Modification:
Cap 1 structure
Poly A Tail:
Yes
Form:
Liquid
Buffer:
1 mM sodium citrate buffer, pH 6.4.
Storage:
Products can be stored at -80°C or below.
We recommend to aliquot the mRNA solution for a better storage. Avoid repeated freeze/thaw cycles.
Shipping:
The products are shipped on dry ice and should be avoided for freeze-thaw cycles.
Application:
Reporter Genes
Shipping Conditions:
Dry ice
