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StablePlus™ 2X Fluorescent RT-LAMP Master Mix

Croyez StablePlus™ 2X Fluorescent RT-LAMP Master Mix is an optimized master mix for reverse-transcription loop-mediated isothermal amplification (RT-LAMP) reactions. This product is a dual enzyme system, providing a rapid and sensitive detection in one pot. A fluorescent dye is also supplied with the kit. The LAMP reactions can be monitored through real-time fluorescence detection. The StablePlus™ version contains nucleic acid stabilizing agent to protect the amplified products.
No. Size Price Qty Status
C15034-1ML 1 mL $520.00 Inquiry
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The following procedure is a general guideline for RT-LAMP reaction. To maintain an RNase-free environment, always wear disposable gloves, and use laboratory consumables and water of nuclease-free grade during the whole experiment course.

RT-LAMP reaction set-up:
1.10X LAMP primer mix
Component 10X concentration Final concentration
FIP 16 μM 1.6 μM
BIP 16 μM 1.6 μM
F3 2 μM 0.2 μM
B3 2 μM 0.2 μM
LOOP F 8 μM 0.8 μM
LOOP B 8 μM 0.8 μM

2. An overview of the reaction set-up is listed in the table below. Place all required reagents on ice. Distribute appropriate volumes into each tube before adding template.
Component Amount Final concentration
StablePlus™ 2X Fluorescent
RT-LAMP Master Mix
12.5 μL 1X
10X LAMP primer mix 2.5 μL 1X
50X LAMP Fluorescent Dye 0.5 μL 1X
RNA template 1-2 μL variable
Nuclease-Free H2O X μL -
Total reaction volume 25 μL -
* See Usage Notes for additional guidelines on primer/template preparation.
3. Add target RNA template to the detection tube. Gently mix the reaction thoroughly to achieve uniform distribution and avoid making bubbles.
4. Incubate at 65°C for 30-60 min.
5. After LAMP reaction complete, the enzyme can be inactivated by heating at 80°C for 10 min.
6. 
For real-time detection, collect fluorescent data using the SYBR® or FAM channels.