Annexin V-iFluor 594 + 7-AAD Apoptosis Detection Reagent

Croyez fluorescent dye (iFluor 594) conjugated Annexin V is highly purified product. During early apoptosis, cells will translocate membrane phosphatidylserine (PS) from the inner face of the membrane to the cell surface. 7-Aminoactinomycin D (7-AAD) is a fluorescent chemical compound with a strong affinity for DNA. It can be used in flow cytometry to evaluate the cell cycle and cell viability during apoptosis. The product can be used in one-step staining procedure without wash step within 20 minutes.
No. Size Price Qty Status
C08007-K01 25 rxns $120.00 Out of stock
C08007-K02 50 rxns $180.00 Out of stock
C08007-K03 100 rxns $300.00 Out of stock
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  C08007-K01 C08007-K02 C08007-K03
Annexin V-iFluor
1 x 0.125 mL 1 x 0.25 mL 1 x 0.5 mL
7-AAD 1 x 0.125 mL 1 x 0.25 mL 1 x 0.5 mL
10X Binding Buffer 1 x 2 mL 2 x 2 mL 3 x 2 mL
Store at 4°C in the dark.
All reagents are stable for one year under proper storage conditions.
1. Collect 1-5x105 cells in the flow tube by centrifugation.
2. Wash cells in 2 mL cold phosphate-buffered saline (PBS) and collect by centrifugation.
3. Re-suspend cell in 500 μL of 1X Binding Buffer.
4. Add 5 μL of Annexin V-iFluor 594 and 5 μL of 7-AAD, gently mix the cells and incubate for 15-20 minutes at RT in the dark.
5. After incubation, the samples should be kept on ice and perform flow cytometry using filters appropriated for Texas Red
   (Texas Red, corresponding to Annexin V-iFluor 594).