Products

Mouse anti-His tag mAb, clone 14-4

Mouse anti-His tag mAb is specific to 6X His tag which can be applied to several
immunoassays including ELISA, WB, IP, IFA and FACS. This antibody has been
identified in several sources (bacteria, yeast and mammalian cells) of His tag
fusion protein.
No. Size Price Qty
C05002-100UG 100 ug -
C05002-1000UG 1000 ug -
The price does not include shipping fee and tax. ORDER
Product type:
Monoclonal
 
Concentration:
1 mg/mL

Immunogen:
6X His tag fusion protein

Host:
Mouse
 
Reactivity:
Recognizes 6X His tag on either the N- or C-terminus of recombinant fusion protein.

Isotype:
IgG1 kappa

Form:
Liquid
 
Storage buffer:
Phosphate Buffered Saline containing 0.01% thimerosal, pH 7.4.

Storage:
Store at 4°C for at least 6 months.
Store at -20°C for long-term preservation. Avoid repeated freeze/thaw cycles.
Suggestion: Divide antibody into several vials. Keep only vials for usage at 4°C.
 
Application:
ELISA, WB, IP, IFA, FACS
 
Manual: 
Application Dilution factor Final concentration
ELISA 1:5000-20000 0.05-0.2 μg/mL
WB 1:1000-10000 0.1-1 μg/mL
IP 1:500-2000 0.5-2 μg/mL
IFA 1:500-1000 1-2 μg/mL
FACS 1:500-1000 1-2 μg/mL
Note: Application concentration may be various determined by the end user.