Mouse anti-His tag mAb, clone 14-4
Mouse anti-His tag mAb is specific to 6X His tag which can be applied to several
immunoassays including ELISA, WB, IP, IFA and FACS. This antibody has been
identified in several sources (bacteria, yeast and mammalian cells) of His tag
fusion protein.
immunoassays including ELISA, WB, IP, IFA and FACS. This antibody has been
identified in several sources (bacteria, yeast and mammalian cells) of His tag
fusion protein.
Product type:
Monoclonal
Concentration:
1 mg/mL
Immunogen:
6X His tag fusion protein
Host:
Mouse
Reactivity:
Recognizes 6X His tag on either the N- or C-terminus of recombinant fusion protein.
Isotype:
IgG1 kappa
Form:
Liquid
Storage Buffer:
Phosphate Buffered Saline containing 0.01% thimerosal, pH 7.4.
Storage:
Store at 4°C for at least 6 months.
Store at -20°C for long-term preservation. Avoid repeated freeze/thaw cycles.
Suggestion: Divide antibody into several vials. Keep only vials for usage at 4°C.
Application:
ELISA, WB, IP, IFA, FACS
Manual:
Note: Application concentration may be various determined by the end user.
Shipping Conditions:
Blue ice
Monoclonal
Concentration:
1 mg/mL
Immunogen:
6X His tag fusion protein
Host:
Mouse
Reactivity:
Recognizes 6X His tag on either the N- or C-terminus of recombinant fusion protein.
Isotype:
IgG1 kappa
Form:
Liquid
Storage Buffer:
Phosphate Buffered Saline containing 0.01% thimerosal, pH 7.4.
Storage:
Store at 4°C for at least 6 months.
Store at -20°C for long-term preservation. Avoid repeated freeze/thaw cycles.
Suggestion: Divide antibody into several vials. Keep only vials for usage at 4°C.
Application:
ELISA, WB, IP, IFA, FACS
Manual:
| Application | Dilution factor | Final concentration |
| ELISA | 1:5000-20000 | 0.05-0.2 μg/mL |
| WB | 1:1000-10000 | 0.1-1 μg/mL |
| IP | 1:500-2000 | 0.5-2 μg/mL |
| IFA | 1:500-1000 | 1-2 μg/mL |
| FACS | 1:500-1000 | 1-2 μg/mL |
Shipping Conditions:
Blue ice
